Spawning Catfish With the Injection of Pituitary
Basically
spawning catfish with pituitary injection equal to the spawning natural way.
Both of readiness parent, pool and handling conditions. The difference lies in
the pituitary injection process in the parent fish before spawning process is
done. Injection process applied to both male and female parent. Injections of
pituitary function to stimulate the growth and maturation of the egg. So that
the results will be better than ever with the naturally spawning catfish.
Pituitary glands obtained from donor fish,
catfish can or adult carp. Try fish donors have equal weight with the weight of
the parent. For example, for a parent with a weight of 750 grams of fish seek
donor with the same weight. This is to ensure that the parent fish pituitary
obtain the proper dosage.
How to get the pituitary gland is to divide
the fish head. Here's how to get the pituitary gland of catfish. The equipment
required is a knife, forceps, tweezers, or a glass test tube, glass grinding
and injection. For the record, all of the equipment used must be clean even
better if sterile and hands must be clean.
1.
First
cut the fish at the base of the head (eg, neck in humans) with a clean knife.
2.
Place
the catfish's mouth pointing upwards, open mouth catfish and sides opening
mouth with a knife so that the head of the fish transversely split into upper
and lower parts. Take the top and remove from the blood.
3.
Discard
bones pituitary cover with forceps, lift the pituitary gland. White granular
glands.
4.
Grind
the pituitary gland with a glass muller, dilute with water aquadestilata 2 ml.
5.
Move
pituitary has been mixed with water in the tube, shake it for 2-3 minutes.
After that let stand for 5 minutes. Liquids will separate, bottom sediments and
form the top layer of clear liquid.
6.
Take
the clear liquid with a syringe. Pituitary ready to be injected in the parent
spawning catfish.
Injecting
the parent catfish done on the back. Way, take sires a hood with a damp cloth.
Injecting the back muscles with a 30o-60o slope of the tail 1.5-2.5 cm deep.
Inject slowly, after all the fluid runs out remove the syringe then massage the
back muscles in order to spread the liquid evenly.
Male
and female parent input that has been injected into the spawning pond.
Furthermore catfish spawning process by injecting the same as the natural
spawning process.
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